A novel single pulsed electromagnetic field stimulates the osteogenesis and angiogenesis ---- in vitro and in vivo study

+ Yin-Chih Fu , Je-Ken Chang, Chung-Hwan Chen, Gwo-Jaw Wang, Mei-Ling Ho +Kaohsiung Medical University, Kaohsiung, Taiwan [email protected] ABSTRACT INTRODUCTION: Pulsed electromagnetic field (PEMF) has been successfully applied for accelerating fracture repair as early as 1979. Since the treatment required 8-12 hrs of application daily, the inconvenience leads us to develop a newly designed single pulsed electromagnetic field (SPEMF). This device is capable of enhancing osteogenesis with human bone marrow derived mesenchymal stem cells (hBMSCs) with 3 min/day treatment. In this study, thymidine incorporation was used to examine cell proliferation. Alkaline phosphatase activity assay and mineralization assay were used to evaluate osteogenic differentiation of hBMSCs and previous bone graft mice model to confirm in vitro study. Our results showed that the cell proliferation was enhanced in SPEMF-treated groups at day 2 and 4 of treatment. The ALP activity of hBMSCs was significantly increased in SPEMF-treated groups after 2-5 days of treatment. The mineralization was also increased after 15-25 days of SPEMF stimulation. Furthermore, a short-term treatment to hBMSCs with SPEMF for 7 days also achieved the same effect of SPEMF stimulation at 15